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KMID : 0613820100200070999
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Journal of Life Science
2010 Volume.20 No. 7 p.999 ~ p.1004
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β-Glucosidase Recovery from a Solid-State Fermentation System by Aspergillus niger
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Chandra M. Subhosh
Reddy B. Rajasekhar
Choi Yong-Lark
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Abstract
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Investigations were carried out on a β-glucosidase produced by Aspergillus niger under solid-state fermentation conditions as a model of enzyme recovery from fermented wheat bran. The leaching efficiency of distilled water to recover the enzyme from the fermented bran was higher than acetate buffer, citrate buffer, citrate-phosphate buffer and 5% methanol; thus, the conditions were further optimized with distilled water as the extracting agent. After fermented bran was washed three times with distilled water for 1.5 hr each under shaking conditions at 1:5 solid to solvent ratio, a maximum recovery of 0.025 U/g of wheat bran was obtained.
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KEYWORD
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β-Glucosidase, wheat bran, solid-state fermentation, Aspergillus niger, recovery
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